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. 2022 Nov 27;5:1301. doi: 10.1038/s42003-022-04264-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Integrated UMAP of scRNAseq data for all conditions. b Bar chart depicting the percentage of cells per cluster in each sample. c Scaled gene expression for MFib1 in control, TGFβ1, and TGFβ1 + GSK343 treated organoids. d Organoids were pre-treated with the EZH2 inhibitor GSK343 for 1 h prior to treatment with TGFβ1 for 48 h. Immunostaining of αSMA and periostin (POSTN) in TGFβ1−, and TGFβ1 + GSK343-treated organoids. Scale bar 150 µm. Images are representative of three independent experiments. e αSMA/DAPI area and f POSTN/DAPI area in TGFβ1 and TGFβ1 + GSK343 treated organoids. Each symbol represents the mean of 18 randomly imaged fields, taken from one organoid per condition, from three independent experiments. Data are presented as the mean ± SEM. **P ≤ 0.01 (αSMA/DAPI area = P ≤ 0.0083; POSTN/DAPI area = P ≤ 0.0017).