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. 2022 Nov 27;5:1301. doi: 10.1038/s42003-022-04264-1

Fig. 6. De novo clusters in response to TGFβ1 represent a “fibrotic” regulon, enriched with motifs for SMAD3 and Fos/Jun.

Fig. 6

a Motif-centric footprinting showing enrichment for SMAD3. b Motif activity at accessible regions in MFib1 treated with TGFβ1 or TGFβ1 + GSK343, compared to Fib2 (Control). AP1 motif enrichment in myofibroblasts is decreased by inhibition of EZH2. c, d Motif-centric footprinting showing enrichment for factors associated with a “fibrotic” regulon; Shown are representative SCENIC-UMAPs of the regulons from the top enriched motifs (right hand panels), and the correspondence with scATACseq enriched motifs (centre panels). e Differentially regulated transcription factor networks (“regulons”) associated with AP1 complex, ETS family, and other fibrosis-associated transcription factors in MFib1 compared to its parent cluster Fib 2. f Blended UMAPs of transcription factor regulon for FOSL2 and JUNB identified by SCENIC in MFib1 and S1.