FIGURE 1.

The zinc fingers and homeoboxes 2 (Zhx2) gene is expressed in hepatocytes and nonparenchymal cells and efficiently deleted in knockout mouse livers. (A) The mouse Zhx2 gene spans 145 kb and consists of 4 exons, including a 2.7‐kb exon 3 that encodes the entire 836 amino acid of Zhx2 protein; loxP sites (red triangles) are used for deletion of flanked exon 3. (B) Immunohistochemical (IHC) staining of adult livers with anti‐Zhx2. Adult mouse liver sections from homozygous Zhx2 ^wt mice, Zhx2 ^Δliv mice (Zhx2 ^fl/fl , Alb‐Cre ⁺ ), and Zhx2 ^KO mice (Zhx2 ^fl/fl , βactin‐Cre ⁺ ) were stained with Zhx2 antibodies and counterstained with hematoxylin. Magnification, ×20.