FIGURE 2.

The absence of Zhx2 in Zhx2 ^KO mice completely blocks DEN‐induced HCC. (A) Male and female Zhx2 ^wt and Zhx2 ^KO mice (9–15 mice/cohort) were given a single intraperitoneal injection of diethylnitrosamine (DEN; 10 mg/kg; Sigma‐Aldrich) or vehicle (phosphate buffered saline [PBS]) at p14. After 9 months, livers were removed, and tumors were quantitated. Representative livers are shown; tumor incidence data are given in Table 1. (B) The maximal tumor size (left panel) and the average number of visible tumors (right panel) in Zhx2 ^wt male mice at 9 months (n = 14) and 10 months (n = 10) and Zhx2 ^KO male mice at 9 months (n = 11) and 10 months (n = 11) after DEN treatment.