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. 2022 Oct 4;6(12):3550–3562. doi: 10.1002/hep4.2106

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© 2022 The Authors. Hepatology Communications published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Cytochrome P450 2E1 (CYP2E1), Pold2 expression, and phosphorylated histone variant H2AX (γH2AX) staining are the same in Zhx2 ^wt and Zhx2 ^KO mice 24 hours after DEN treatment. Data were obtained from p14 Zhx2 ^wt and Zhx2 ^KO mice before (0 h) and 4, 8, or 24 h after DEN treatment. (A,B) Hepatic Cyp2e1 (A) and Pold2 (B) mRNA levels were quantitated by real‐time quantitative PCR and normalized to ribosomal protein L30. (C,D) Hepatic CYP2E1 (C) and Zhx2 (D) protein levels were analyzed by western blot; each lane represents an individual mouse. Blots were reprobed with antibodies against GAPDH. (E) Representative images from liver sections after IHC staining for γH2AX and counterstained with hematoxylin.