FIGURE 5.

Cell proliferation is reduced in the livers of Zhx2 ^KO mice compared with Zhx2 ^wt mice during the 7 days after DEN treatment. (A) Liver sections were obtained from p14 mice before (0 h) and 8 h, 1 day, 2 days, and 7 days after DEN treatment and stained for the proliferation marker Ki67. Representative images from liver sections at designated timepoints are shown. (B) Quantitation of Ki67 IHC staining. Aperio ScanScope XT digital slides scanner was used to scan the entire stained slide at ×20 magnification to create a single high‐resolution digital image. Quantification of nuclear staining for Ki67 was done using HALO imaging software (Indica Labs). Data were from three mice in each group. *p < 0.05. (C) Hepatic interleukin‐6 (IL‐6) and tumor necrosis factor α (TNFα) mRNA levels were quantitated by real‐time quantitative PCR and normalized to ribosomal protein L30. **p < 0.01. (D–F) Immunoblotting and densitometry of phosphorylation of AKT (pAKT; D), AKT serine/threonine kinase 1 (AKT1), AKT2, nuclear factor kappa B (NF‐κB) p65, and heat shock protein 90 (HSP90) (E,F) from the 7‐day livers of PBS‐treated or DEN‐treated Zhx2 ^KO mice or Zhx2 ^wt mice. *p < 0.05 and **p < 0.01 (n = 4).