TABLE 1.
Sequences of primers used in this study
| Species and product | Primer sequence | Product size (bp) | No. of PCR cycles | % Agarose gel | Reference |
|---|---|---|---|---|---|
| Human | |||||
| G3PDHa | 5′-TGAAGGTCGGAGTCAACGGATTTGGT-3′ | 983 | 16 | 1.5 | |
| 5′-CATGTGGGCCATGAGGTCCACCAC-3′ | |||||
| MCP-1 | 5′-CCCAGGGGTAGAACTGTGGTTCAA-3′ | 480 | 34 | 1.1 | 24 |
| 5′-CTCGCTCAGCCAGATGCAATCAAT-3′ | |||||
| RANTES | 5′-CGCTGTCATCCTCATTGCTA-3′ | 197 | 30 | 0.8 | 17 |
| 5′-CACACACTTGGCGGTTCTT-3′ | |||||
| Mouse | |||||
| G3PDH | 5′-CCATGGAGAAGGCTGGGG-3′ | 194 | 25 | 1.5 | 12 |
| 5′-CAAAGTTGTCATGGATGACC-3′ | |||||
| MCP-1 | 5′-AGCCAACTCTCACTGAAGCCA-3′ | 444 | 31 | 1.1 | 12 |
| 5′-CTACACAGAAGTGCTTGAGGTGGT3′ | |||||
| RANTES | 5′-ACCTGCCTCCCCATATGGCT-3′ | 200 | 32 | 2.0 | 6 |
| 5′-GTATTCTTGAACCCACTTCTTC3′ |
a
Primers purchased from Clontech Laboratories, Palo Alto, Calif.