. 2022 Nov 28;257(1):6. doi: 10.1007/s00425-022-04036-5

Table 2.

M. polymorpha-Trichoderma colonization on in vitro culture

Trichoderma specie	Plant			Fungi			Ratio³
Trichoderma specie	Ct	SD	Qty¹	Ct	SD	Qty²	Ratio³
T6	19.38	0.08	2.35	23.07	0.12	0.42	0.18 ± 0.04^a
T11	18.72	0.13	2.98	22.61	0.25	1.01	0.34 ± 0.01^b
T25	19.63	0.02	2.11	23.07	0.18	0.42	0.20 ± 0.03^a
T34	19.17	0.19	2.55	23.08	0.14	0.41	0.16 ± 0.06^a
T77	19.37	0.12	2.36	22.72	0.22	0.87	0.37 ± 0.05^b
T87	19.32	0.04	2.41	21.84	0.19	2.00	0.83 ± 0.07^c
T120	18.72	0.16	2.98	21.45	0.05	2.50	0.84 ± 0.14^c
T123	19.08	0.09	2.63	21.18	0.24	2.45	0.93 ± 0.05^c

Trichoderma species T. parareesei (T6), T. atroviride (T11), T. asperellum (T25), T. harzianum (T34), T. koningii (T77), T. virens (T87), T. brevicompactum (T120), T. hamatum (T123) were used

Quantification of Trichoderma DNA in M. polymorpha was performed by qPCR

¹Quantity of M. polymorpha DNA (ng) referred to EF1 gene

²Quantity of Trichoderma DNA (ng) referred to Actin gene

³Proportion of fungal DNA vs. plant DNA. Values are the means of three plant-pools (eight plants each one) from three independent experiments with the corresponding standard deviations. One-way analysis of variance (ANOVA) was performed, followed by the Tukey’s test. Different letters represent significant differences (P < 0.05)