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. 2022 Nov 27;12:20388. doi: 10.1038/s41598-022-23941-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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T. cruzi epimastigotes proliferation and viability in the presence of FEX. (A) Epimastigote proliferation was strongly inhibited for up to 72 h of treatment. (B) The percentage of parasites after 72 h in the presence of the drug (i.e., 96 h of growth) indicates that the most effective concentrations were 30, 40, and 50 µM. (C) After removal of the drug from the medium, the percentage of parasites indicates an increase in the number of epimastigotes. (D) Epimastigote viability after 72 h of treatment. The percentage of viable parasites was similar, except in the presence of 50 µM. The data are the average of three independent experiments in triplicate (***p < 0.001).