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. 2022 Nov 27;12:20388. doi: 10.1038/s41598-022-23941-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Epimastigotes cell cycle after removal of FEX from the medium. (A) Histograms of control and treated parasites. Black and color lines indicate untreated and previously treated epimastigotes, respectively. (B) Percentage of parasites in G1, S, and G2/M. The main difference occurred after treatment with 40 and 50 µM when there was a great change in the percentage of parasites in the G1 and G2/M phases. The histograms are representative and the data are the average of two independent experiments (*p > 0.05; **p < 0.05; ***p < 0.001).