FIGURE 3.

The aberrant expression of FXR affects the glycolysis of colon cancer. (A) GSEA results implied that the down‐regulation of FXR is remarkably correlated with Glycolysis/Gluconeogenesis. (B) Pyruvic acid, lactic acid, citric acid and malic acid were measured in SW620 treatment groups. (C) Pyruvic acid, lactic acid, citric acid and malic acid were measured in HCT116 treatment groups. (D–E) Seahorse XP 96 was used to analyze ECAR and OCR of SW620 and HCT116. The glycolysis level, the basic oxygen consumption rate of glycolysis, and the maximum oxygen consumption rate were calculated based on the test results. During the detection of Seahorse XP 96, glucose, oligomycin, carbonyl cyanide m‐chlorophenyl hydrazone (CCCP), rotenone and 2‐deoxyglucose (2DG) were sequentially injected into the experimental microchamber. * p < 0.05