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. 2022 Nov 14;12:980974. doi: 10.3389/fcimb.2022.980974

Figure 4.

Figure 4

Validation and characterization of HCMV circUL55. (A) Inverse RT-PCR products of circUL55 with BSJs validation primer set in Figure 1 . The 189 bp band that amplified from circUL55_1 (685 nt) was the predicted amplicon of the primer. A 340 bp band from circUL55_2 (836 nt) was amplified. Linear GAPDH and linear UL123 were amplified as RNase R-resistant control. (B) Sanger sequencing result of circUL55_1 and circUL55_2 BSJs. (C) Isoforms of circUL55mapping to HCMV UL55 gene.