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. 2022 Nov 14;12:980974. doi: 10.3389/fcimb.2022.980974

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Copyright © 2022 Deng, Wang, Zhang, Ma, Qi, Liu, Li, Ruan and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation and characterization of HCMV circUL89. (A) Inverse RT-PCR products of circUL55 with BSJs validation primer set in Figure 1 . A 189 bp band was achieved as antipicated. Linear GAPDH and linear UL123 were amplified as RNase R-resistant control. (B) Sanger sequencing result of circUL89 BSJ. (C) Inverse RT-PCR results of HCMV circUL89 fragments A and B with divergent outer primer sets indicated in Figure 1 . Both of the two fragments, which were overlapped in two terminals, were about 2000 bp in size. The whole sequence of circUL89 was achieved by sequencing and splicing. (D) The circUL89 full sequence mapping to HCMV UL89 gene. (E) Northern blot of circUL89.