FIG. 2.

Induction of DPPIV activity in HGF stimulated with various stimulants. Confluent HGF (donor, MH-12) were stimulated with IL-1α (10 ng/ml), PMA (100 ng/ml), IFN-γ (200 U/ml), TNF-α (40 ng/ml), or E. coli LPS (10 μg/ml) for 6 days in α-MEM supplemented with 10% FCS in 96-well plates and then washed with PBS three times, and confluent-monolayer cells in the plates were examined by DPPIV assay with Gly-Pro-pNA as a substrate. The results are representative of four different experiments with four different donors (MH-12, NIK, FE, and YS-G). Differences from the control were significant at P < 0.001 (∗∗) and P < 0.01 (∗).