FIG. 3.

Induction of DPPIV activity in HGF stimulated with various bacterial components. Confluent HGF (donor, NIK) were stimulated with various concentrations of bacterial components for 6 days in α-MEM supplemented with 10% FCS in 96-well plates and then washed with PBS three times, and confluent-monolayer cells in 96-well plates were examined by DPPIV assay with Gly-Pro-pNA as a substrate. E. coli LPS extracted with phenol-water, P. gingivalis (P.g.) LPS extracted with phenol-water, P. intermedia (P.i.) LPS extracted with phenol-water (PW), P. intermedia LPS extracted with PCP, PGP, and P. gingivalis fimbriae were used. The results are representative of two different experiments with two different donors (NIK and AK-G). Differences from the control were significant at P < 0.01 (∗∗) and P < 0.05 (∗).