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Inhibition of CD26 mRNA accumulation in HGF by CHX. HGF (donor, NIK) were preincubated with or without CHX (10 μg/ml) for 2 h and stimulated with or without IL-1α (10 ng/ml) for 24 h, and then the total RNA was extracted for real-time quantitative PCR. The relative difference in CD26 mRNA expression was calculated according to the Ct value as described in Materials and Methods. The relative value of mRNA expression at day 0 was converted to 100%.
