TABLE 1.
Expression of CD26 (DPPIV) and CD14 on human fibroblasts of various organsa
| Fibroblasts | Origin | % Positive cells (mean
fluorescence value)
|
|
|---|---|---|---|
| CD26 | CD14 | ||
| MH-12 | Gingiva | 32.5 (15.0) | 56.1 |
| KEK | Gingiva | 32.9 (11.1) | 42.3 |
| NIK | Gingiva | 5.2 (5.6) | 61.3 |
| YS-G | Gingiva | 22.6 (5.7) | 69.9 |
| AK-G | Gingiva | 5.2 (4.2) | 58.6 |
| FE | Gingiva | 26.1 (8.4) | NDb |
| AK | Periodontal ligament | 19.7 (3.8) | 24.9 |
| TT | Periodontal ligament | 7.7 (5.2) | ND |
| YS | Periodontal ligament | 11.1 (7.9) | 14.6 |
| KS | Periodontal ligament | 18.1 (4.5) | 10.9 |
| IMR-90 | Lung | 96.9 (51.9) | 1.1 |
| WI-38 | Lung | 88.1 (94.5) | 3.9 |
| MRC-5 | Lung | 99.2 (95.4) | 0.5 |
| FS-4 | Skin | 87.2 (88.4) | 0.7 |
| SF-MA | Skin | 80.9 (96.8) | 1.9 |
a
Fibroblasts derived from various organs were maintained in α-MEM supplemented with 10% FCS. Cells were collected from confluent monolayers with trypsin-EDTA, and CD26 and CD14 antigens on the cell surface were assessed by flow cytometry with indirect staining as described in Materials and Methods. Isotype-matched Abs were used as negative controls.
b
ND, not done.