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IFN-γ produced when endogenous IL-10 is blocked is IL-12 dependent. BALB/c spleen cells were cultured with various stimuli in the presence of anti-IL-10, anti-IL-12, or both antibodies (10 μg/ml). Supernatants were collected at 72 h and analyzed for IFN-γ by ELISA. The results from one of two similar experiments are shown.
