FIG. 6.

B. abortus and bDNA-induced IL-12 are enhanced by IFN-γ priming, especially in the presence of anti-IL-10. LPS only induced detectable IL-12 with the combination of IFN-γ addition and anti-IL-10. Spleen cells from IFN-γ KO mice (BALB/c background) were stimulated with B. abortus, LPS, or bDNA in the presence of IFN-γ (50 ng/ml) and/or anti-IL-10 (10 μg/ml). LPS, bDNA, or B. abortus was added to the culture 10 min after the addition of anti-IL-10 and IFN-γ. IL-12 p70 was measured by ELISA 18 h later. The results of one of three representative experiments are shown. The results in normal BALB/c mice were similar.