TABLE 1.
IL-12 production 3 h after intravenous injection of bacterial constituents in BALB/c micea
| Treatment | IL-12 produced (pg/ml)
|
|
|---|---|---|
| Expt 1 | Expt 2 | |
| PBS | 35.7 ± 9.8 | 8.5 ± 0.6 |
| B. abortus | 234 ± 68 | 467 ± 5.4 |
| B. abortus DNA | 104 ± 32.8 | 203 ± 40 |
| B. abortus LPS | 9.7 ± 3.0 | 17.3 ± 2.9 |
| E. coli DNA | ND | 107 ± 27.5 |
| B. abortus DNA (low dose) | 9.9 ± 3.6 | ND |
a
BALB/c mice were injected with B. abortus (108 organisms), B. abortus LPS (30 μg), B. abortus DNA or E. coli DNA (100 μg), or low dose B. abortus DNA (0.1 μg). Three hours after injection, spleens were removed and cultured individually without further stimulation. Supernatants were harvested at 24 h and assayed by ELISA for IL-12 p40. Spleens removed 8 and 24 h after injection produced less IL-12 than that seen at 3 h, although the relative potency of constituents remained the same (data not shown). The results reflect the means ± the standard error for three mice/treatment. ND, not determined.