FIG. 3.

LTR72 enhances local lymph node Th2-type responses to nasally delivered Pa. BALB/c mice were immunized twice (weeks 0 and 4) i.n. with a two-component Pa consisting of rPT and FHA with or without LTR72 (1 μg) and with or without light anesthesia (Anes.). At 6 weeks, thoracic lymph node (LN) cells (A) and superficial cervical LN cells (B) were prepared, and proliferation and cytokine assays were performed as described in the legend to Fig. 1. ∗ and +, P < 0.05 and P < 0.01, respectively, versus Pa alone (by Student's t test).