Table 1.

SPR analysis of the binding affinity and pH-dependency of G532 and G532^Ctrl

	Human EGFR			Mouse EGFR
	KD (M) pH 6.5	KD (M) pH 7.4	KD ratio (pH 7.4/6.5)	KD (M) pH 6.5	KD (M) pH 7.4	KD ratio (pH 7.4/6.5)
G532	2.94E-07	3.90E-06	13.262	5.47E-07	1.81E-06	3.309
G532Ctrl	4.06E-07	2.43E-07	0.599	1.27E-06	7.43E-07	0.586

The dissociation constant (KD) values were calculated with a steady-state affinity model using Biacore T200 evaluation software.