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. 2022 Aug 28;14(6):1295–1310. doi: 10.1016/j.jcmgh.2022.08.008

Figure 3.

Figure 3

High- and low-passage IEOs are functionally similar. (A) Outline of experimental design. (B) Bright-field images of TI- and sigmoid colon–derived IEOs at low and high passage taken by the EVOS FL system (Life Technologies). Scale bars: 300 μm. (C) Comparison of growth curves between low- and high-passage TI- and SC-derived IEOs (n = 2, 6 technical replicates per each biological replicate). (D) Representative images of TI organoids in standard medium Non-treated (NT), vehicle control medium (DMSO), cultured with forskolin IFNγ+forskolin or TNFα+forskolin taken with by Incucyte. Scale bars: 800 μm. (E) Comparison of organoid size between early and late-passage organoids after proinflammatory cytokines and forskolin treatments (n = 2, 4 technical replicates per each biological replicate).