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. 2022 Aug 28;14(6):1295–1310. doi: 10.1016/j.jcmgh.2022.08.008

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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High- and low-passage IEOs are similar upon differentiation and proinflammatory cytokine stimulation. (A) High- and low-passage IEOs look similar before and after differentiation. Bright-field images of TI- and sigmoid colon (SC)-derived IEOs after in vitro differentiation, respectively, taken by the EVOS FL system (Life Technologies). Scale bars: 300 μm. Outline of experimental design. (B) Gene expression of selected differentiation markers in IEOs by gut segment and passage (high or low). (C) Venn diagram illustrating overlap of differentially expressed genes in high- and low-passage IEOs upon differentiation and co-culture with IFNγ or TNFα. (D) Bright-field images of TI-derived IEOs at low and high passage after proinflammatory cytokine treatment (TNFα or IFNγ), taken by the EVOS FL system (Life Technologies). Scale bars: 300 μm. D, Differentiated; UD, Undifferentiated.