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. 2022 Aug 28;14(6):1295–1310. doi: 10.1016/j.jcmgh.2022.08.008

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Genes are differentially expressed with IEO passage. (A) Representative CpGs and genes showing DNAm and mRNA expression associated significantly with passage in cohort 1 and a validation cohort. Samples are colored by passage number and grey lines connect samples derived from the same patient. Regression lines between passage and DNAm/expression are in black. (B) mRNA expression of representative genes showing passage-dependent differences in response to in vitro differentiation, IFNγ, or TNFα. D, Differentiated; UD, Undifferentiated; UT, Untreated.