Genes are differentially expressed with IEO passage. (A) Representative CpGs and genes showing DNAm and mRNA expression associated significantly with passage in cohort 1 and a validation cohort. Samples are colored by passage number and grey lines connect samples derived from the same patient. Regression lines between passage and DNAm/expression are in black. (B) mRNA expression of representative genes showing passage-dependent differences in response to in vitro differentiation, IFNγ, or TNFα. D, Differentiated; UD, Undifferentiated; UT, Untreated.