Table 2.

Differentiation Medium Components

Differentiation medium (total volume, 12.5 mL)	Final concentration
Advanced DMEM/F12+++	97.44% (vol/vol)
Primocin (Invivogen San Diego, CA)	500 μg/mL
B-27 R supplement (Invitrogen, Carlsbad, CA)	1×
N-acetylcysteine (Sigma, St. Louis, MO)	1.25 mmol/L
A3801 (Tocris, Bristol, UK)	500 nmol/L
SB202190 (Sigma)	10 μmol/L
Murine EGF (Invitrogen)	50 ng/mL
Murine Noggin (Peprotech, Rocky Hill, NJ)	100 ng/mL

EGF, Epidermal Growth Factor; DMEM, Dulbecco’s modified Eagle medium.