Skip to main content
. 2022 Aug 2;14(6):1311–1331. doi: 10.1016/j.jcmgh.2022.07.011

Figure 14.

Figure 14

Supplying GluCer restores cell proliferation in mTORC2 KO mice liver. (A) Representative immunohistochemistry images for the Ki-67–positive cells in GluCer-treated Ctrl and R-LKO mice after PH. Right: Graph shows the quantification of Ki-67–positive cells/total cells at the indicated time points after PH. Means ± SD, 5 mice per group. (B) Western blot analysis of hepatic PCNA expression in GluCer-treated Ctrl and R-LKO mice at 36 hours after PH. Quantifications were normalized to β-tubulin. Means ± SD, 4 mice per group. (C) LW/BW of GluCer-treated control (Ctrl) and R-LKO mice 36 hours after PH (3–4 mice for each time point). (D) Triglyceride levels in GluCer-treated Ctrl and R-LKO mice liver 36 hours after PH (3–4 mice for each time point). (E) FA levels in GluCer-treated Ctrl and R-LKO mice liver 36 hours after PH (3 mice for each time point). (F) Western blot analysis of hepatic PCNA expression in myriocin-treated Ctrl and R-LKO mice at 36 hours after PH. Quantifications were normalized to β-tubulin. Means ± SD, 3 mice per group. (G) LW/BW of vehicle and myriocin-treated mice 36 hours after PH (4–5 mice for each time point).