Extended Data Fig. 6 ∣. Ligand independence of the interaction between hpMR1 and TAPBPR by SPR.

a, SPR sensorgrams of varying concentrations of empty or ligand loaded hpMR1/bβ₂m flowed over a streptavidin chip coupled with TAPBPR^WT-biotin. The analyte concentrations are noted on the first three sensorgrams and are the same for every experiment. b, SPR sensorgrams of varying concentrations of Ac-6-FP loaded hMR1/hβ₂m wild-type or hMR1 C262S/hβ₂m flowed over a streptavidin chip coupled with TAPBPR-biotin. The analyte concentrations are noted on the sensorgram. The RU vs hMR1 concentration is also shown with fitting shown in the dotted lines. Data are mean ± SD for n = 3 technical replicates. The C262S mutation is a Cys->Ser mutation for the free hMR1 Cys on the α3 domain.