Table 3.

Most important B. mallei typing techniques and relevant references of the methods or examples for application.

Typing method	Technique	Targets	Remark	References
MLST	PCR	Housekeeping genes	Low discriminatory power: B. mallei assigned to ST40 or ST100	(4)
MLVA	PCR	Tandem repeats	Useful for differentiation among strains of one outbreak, but targets are prone to homoplasy	(38, 39)
HRM-PCR	PCR	Canonical SNPs	Three large genotype groups and several subgroups can be differentiated	(45, 62, 63)
cgMLST	WGS	Nucleotide differences in core genome genes	In-depth differentiation based on assemblies	(31, 59)
cgSNP	WGS	Single nucleotide differences in core genome including intergeneric regions	In-depth differentiation based on sequencing reads or assemblies	(41, 59)