Fig. 3.

Adrenal LDs. A: Oil Red O staining of adrenals in control (n = 4) and seipin KO mice (n = 4). Scale bars represent 100 μm. B: Total cholesterol (TC), free unesterified cholesterol (FC), and CE contents in control and seipin KO mice adrenals. C: TAG content in control and seipin KO mice adrenals. Total lipids were extracted from the adrenals of control (n = 7) and seipin KO male mice (n = 7) using the Folch method. Total cholesterol, unesterified cholesterol, CEs, and TAG were assayed as described in the Materials and methods section. Data are expressed as mean ± SEM. ∗P < 0.05.