Fig. 5.

Corticosterone production in control and seipin KO mice. A: Corticosterone production in control and seipin KO male mice. Male control (n = 8) and seipin KO mice (n = 8) were treated with cosyntropin (2.5 IU per mouse, 1 h) and serum collected before and 1 h after the treatment. B: Corticosterone production in control and seipin KO female mice. Female control (n = 8) and seipin KO mice (n = 8) were treated with cosyntropin (2.5 IU per mouse, 1 h) and serum collected before and 1 h after the treatment. C: Ex vivo corticosterone production in male control and seipin KO mice (n = 8). Adrenals from male mice were cut in half and cultured ex vivo. After 0.5 h, the media were changed with fresh media with 2.5 mM Bt₂cAMP in the absence or presence of h-HDL₃ (500 μg cholesterol/ml). After 1 h incubation, corticosterone levels in the media were measured. D: Ex vivo corticosterone production in female control (n = 8) and seipin KO mice (n = 8). Adrenals from female mice were cut in half and cultured ex vivo. After 0.5 h, the media were changed with fresh media with 2.5 mM Bt₂cAMP in the absence or presence of h-HDL₃ (500 μg cholesterol/ml). After 1 h incubation, corticosterone levels in the media were measured. Data are expressed as mean ± SEM. ∗P < 0.05; ∗∗P < 0.01.