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IHC expression and FISH results. Nuclear beta-catenin expression (A, yellow arrow), GAB1 (B) and SFRP1 (C) expression was seen. NPR3 (D) and KCNA1 (E) was seen for Group 3 and 4. FISH shows MYCN amplification (F), specimens containing either more than 10 signals or innumerable tight clusters of signals (F, blue arrow) in more than 10% of tumor cells were considered of amplification
