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. 2022 Jul 4;31(23):3987–4005. doi: 10.1093/hmg/ddac083

Figure 3.

Figure 3

CHCHD2-T61I is expressed in different regions of the brain and localizes to the mitochondrial IMS. (A) Representative immunoblots of RIPA-soluble lysate from the cortex (CTX), midbrain (SN), spinal cord (SC) and hippocampus (HIP) from 1-year-old CHCHD2-T61I Tg mice and nontransgenic WT littermates. Exogenous CHCHD2 expression is clearly seen in all regions, with the highest expression seen in the cortex, midbrain and hippocampus. (B) Representative images of whole brain horizontal slices of 1-year-old CHCHD2-T61I Tg line U144, line U105 and WT littermates immunostained for M2 (flag) and CHCHD2 (Scale bar: 2 mm). (C) Representative images of cortex and midbrain regions immunostained for M2 (flag) and CHCHD2 from 1-year-old CHCHD2-T61I Tg line U144, line U105 and WT littermates (Scale bar: 20 μm). (D) Representative immunoblots of mitochondrial subfractionation from whole brains of 3- to 4-month-old CHCHD2-T61I Tg line U105 mice and WT littermates (n = 4 mice/genotype). The exogenous CHCHD2-T61I and endogenous CHCHD2 target correctly to the mitochondrial IMS in vivo.