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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: Methods Enzymol. 2018 Feb 21;601:111–144. doi: 10.1016/bs.mie.2017.11.028

Fig. 1.

Fig. 1

Steps in building and sequencing an amplicon library of DSB repair events. First, linear or circular DNA with an inducible break site must undergo a break and be given time for repair. The sequence surrounding the break site (shown in green) may experience an error such as an insertion, deletion, or base substitution (shown in red). To capture the frequency and variety of these errors the sequence surrounding the break site is PCR amplified, optionally with primers that contain barcodes. The amplicon products are then sequenced, and the sequenced reads are mapped to the reference sequence. Finally, the mapped reads are analyzed by the Hi-FiBR analysis.