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Top-down targeted MS/MS for protein identification in a selected sSEC fraction. (A) CAD-based MS/MS experiments are performed on selected LC retention time windows containing proteoforms of interest. (B) High-resolution MS demonstrating representative precursor ion isolation and effective CAD-based fragmentation of a 42.9 kDa protein, yielding high-resolution tandem mass spectra. (Adapted and reprinted with permission from [9] Copyright 2017 American Chemical Society.)
