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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Methods Mol Biol. 2022;2500:15–30. doi: 10.1007/978-1-0716-2325-1_3

Fig. 6.

Fig. 6

Visualizing and verifying fragment ions against an imported protein sequence in MASH Explorer. A) Imported database search results can be found under the Protein Search Result tab. B) MASH Explorer’s ‘Protein Sequence Table’ allows users to visualize fragment ion mapping for different MS/MS techniques to characterize the protein sequence and PTMs. The protein amino acid sequence corresponding to the identified protein is directly imported from the database search. Correcting the protein sequence according to the database search (N-terminal methionine excision), results in significantly improved ion fragmentation lists. C) MASH Explorer enables manual verification of MS/MS fragment ion data and adjustment of charge state and monoisotopic mass.