Table 2. RT-qPCR system.
| Reagent | Quantity added per tube |
|---|---|
| 5x RT buffer | 5 µl |
| 10 mM dNTPs | 2 µl |
| Rnasin (40 U/µL) | 0.5 µl |
| M-MLV-RTase (200 U/µl) | 0.5 µl |
| RNase-Free H2O | 6 µl |
| Reagent | Quantity added per tube |
|---|---|
| 5x RT buffer | 5 µl |
| 10 mM dNTPs | 2 µl |
| Rnasin (40 U/µL) | 0.5 µl |
| M-MLV-RTase (200 U/µl) | 0.5 µl |
| RNase-Free H2O | 6 µl |