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. 2022 Nov 25;10:e14369. doi: 10.7717/peerj.14369

Table 3. RT-qPCR reaction system.

Reagent Quantity added per tube
SYBR premix ex taq 6.0 µL
Forward primer (5 µM) 0.5 µL
Downstream primers (5 µM) 0.5 µL
Template (reverse transcription product) 1.0 µL
RNase-Free H2O 4.0 µL