Table 3. RT-qPCR reaction system.
| Reagent | Quantity added per tube |
|---|---|
| SYBR premix ex taq | 6.0 µL |
| Forward primer (5 µM) | 0.5 µL |
| Downstream primers (5 µM) | 0.5 µL |
| Template (reverse transcription product) | 1.0 µL |
| RNase-Free H2O | 4.0 µL |
| Reagent | Quantity added per tube |
|---|---|
| SYBR premix ex taq | 6.0 µL |
| Forward primer (5 µM) | 0.5 µL |
| Downstream primers (5 µM) | 0.5 µL |
| Template (reverse transcription product) | 1.0 µL |
| RNase-Free H2O | 4.0 µL |