Table 1. Schedule of two bottle training and testing for determining taste preference and aversion in rats.

DAYS	Training/Test condition		Animal condition during testing
3	Acclimatization	Food and water provided ad libitum	Non–deprived
1	Two Bottle Training (condition animals to drink from two bottles)	Rats are transferred into the Lickometer setup Test is run for 1 h per group. An empty bottle and one water filled bottle is provided, position is switched every 15 min Food and water is removed overnight	Non–deprived
1	Two Bottle Training (condition animals to drink from two bottles)	Rats are transferred into the Lickometer setup Test is run for 1 h per group. An empty bottle and one water filled bottle is provided, position is switched every 15 min Food and water is removed overnight	Deprived
1	Two Bottle Training (condition animals to drink from two bottles)	Rats are transferred into the Lickometer setup Test is run for 1 h per group An empty bottle and one water filled bottle is provided, position is switched every 15 min Food and water is provided after testing	Deprived
1	Two Bottle Training (condition animals to drink from two bottles)	Rats are transferred into the Lickometer setup Test is run for 1 h per group An empty bottle and one water filled bottle is provided, position is switched every 15 min Food and water is provided after testing	Deprived
2	Rest	Food and water provided ad libitum	Non–deprived
1	Two Bottle preference test (Preferred taste quality)	Sweet taste preference testing Test is run for 1 h per group Sucrose solution is provided as tastant One bottle with tastant and one bottle with water is provided, position is switched every 15 min Food and water is removed overnight	Non–deprived
1	Two Bottle preference test (Aversive taste quality)	Bitter taste preference testing Denatonium solution is provided as tastant One bottle with tastant and 1 bottle with water is provided, position is switched every 15 min Food and water is removed overnight	Deprived
1	Two Bottle preference test (Aversive taste quality)	Salty taste preference testing NaCl solution is provided as tastant One bottle with tastant and one bottle with water is provided, position is switched every 15 min Food and water is provided after testing	Deprived
1	Rest	Food and water provided ad libitum	Non–deprived