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T. gondii tachyzoites from an in vitro culture blotted with sera from individual mice immunized with p1tPASAG1 twice at weeks 0 and 3 (A), once at week 0 (B), and not immunized (C). Sera were tested at week 5 and show recognition of native SAG1. Serum from a mouse infected with T. gondii and the monoclonal antibody S13 recognizing SAG1 were used as positive controls.
