TABLE 1.
Binding of predicted epitopes and frequencies of specific CD8+ T lymphocytes obtained by ELISPOT assay
| Predicted epitope | Binding
(nM)
|
Frequency of responding CD8+ T
lymphocytesa in indicated mouse group
|
|||
|---|---|---|---|---|---|
| Predicted | Observedb | Control (not immunized) | p1tPASAG1 immunized | p1tPASAG1 immunized and RH challenged | |
| AESKSVII (T1) | 110 | 225 | 0 | 1.7 ± 0.9 | 2.4 ± 2.2 |
| QTFVVGCI (T2) | 219 | 160 | 0 | 0 | 0 |
| NEKSFKDI (T3) | 352 | 123 | 0 | 0 | 0.13 ± 0.2 |
| VTGLIGSI (T4) | 402 | >100,000 | 0 | 0 | 0 |
a
Numbers of responders per 105 spleen cells. Values are means ± standard deviations (five mice per group).
b
Affinity-purified Kk was incubated with radiolabelled influenza NP 50-57 as the indicator peptide and increasing concentrations of SAG1 peptide. Binding was determined by gel filtration and expressed as log [IC50 (NP 50-57)/IC50 (PfCSP peptide)]. IC50 of the NP 50-57 peptide was 10 nM.