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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: J Invest Dermatol. 2021 Dec 7;142(7):1882–1892.e5. doi: 10.1016/j.jid.2021.11.027

Figure 1. Low NUMB levels are correlated with decreased overall survival in patients with melanoma.

Figure 1.

(a) KaplaneMeier survival curve analysis of the overall survival of patients with low and high NUMB mRNA expression in melanoma was used to compare a quarter each of the patients having the highest with those having the lowest NUMB expression in their tumor (Cancer Genome Atlas Network, 2015). (b) Immunoblot analysis showing NUMB and NOTCH expression in melanocytes (FOM#1 and #2), primary melanoma cell lines (WM35, WM1366, WM3211), and met melanoma cell lines (WM1799, 1205Lu, WM3918, WM3451) in whole-cell lysates, cytoplasmic fractions, or nuclear fractions. Blotting for b-actin, HSP90, and lamin A/C serves as loading controls. The relative intensities of the immunoblot bands were quantified. (c) Immunofluorescent staining showing NUMB expression in melanoma cell lines. Bar = 100 mm. met, metastatic.