Figure 3. Knockdown of NUMB promotes melanoma metastasis in vivo.
(a) H&E staining of lung tissue sections showing metastatic lesions in in vivo metastasis assays. Mice were injected intravenously with WM1799 melanoma cells transduced with a control shRNA or with shRNAs targeting NUMB. Arrowheads indicate metastasis. Bar = 1 mm. (b) Quantification of the percentage of metastatic area per lung tissue section (n = 10). (c) Quantification of the number of lung metastases per mouse lung section (n = 10). shLuc, short hairpin RNA targeting firefly luciferase; shRNA, short hairpin RNA.