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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: J Invest Dermatol. 2021 Dec 7;142(7):1882–1892.e5. doi: 10.1016/j.jid.2021.11.027

Figure 5. Knockdown of NUMB rescues cell invasion in melanoma cells treated with GSK-3i.

Figure 5.

(a, b) Control WM1799 cells (WM1799 shLuc) or WM1799 cells transduced with shNUMB (WM1799 shNUMB #1 and #2) were grown as spheroids, embedded in 3D collagen gel, and allowed to invade with or without GSK-3i IX at 3 mM for 11 days. Control WM3451 cells (WM3451 shLuc) or WM3451 cells transduced with shNUMB (WM3451 shNUMB #2) were grown as spheroids, embedded in 3D collagen gel, and allowed to invade with or without the presence of GSK-3i IX at 3 mM for 8 days. Bars = 200 mm. 3D, three-dimensional; GSK- 3i, glycogen synthase kinase-3 inhibitor; n.s., not significant; shLuc, short hairpin RNA targeting firefly luciferase; shNUMB, short hairpin RNA targeting NUMB.