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. Author manuscript; available in PMC: 2022 Nov 28.
Published in final edited form as: J Invest Dermatol. 2021 Dec 7;142(7):1882–1892.e5. doi: 10.1016/j.jid.2021.11.027

Figure 6. The effects of NUMB knockdown on CCNE and MITF expression.

Figure 6.

(a, b) Quantitative real time-PCR showing the expression of CCNE and MITF in the metastatic melanoma cell lines (a) WM1799 and (b) WM3451 transduced with shNUMB #1 and #2 compared with that in shLucs. mRNA levels of CCNE and MITF were normalized to that of GAPDH. (c) The potential mechanism of NUMB to regulate invasion activity in melanocyte and melanoma through the NUMB–NOTCH–CCNE axis. n.s., not significant; shLuc, short hairpin RNA targeting firefly luciferase; shNUMB, short hairpin RNA targeting NUMB.