Figure 3.

Knockdown of circFARSA inhibited tumor growth and metastasis in vivo. BALB/c nude mice have subcutaneously injected with NCI-1299 (1 × 10⁶) cells with stably low-expressing circFARSA (sh-FARSA) or sh‑NC. (a), Tumors were dissected and imaged. (b), Histology examination of tumor slices by hematoxylin and eosin (H&E) staining, Scale bar = 50 μm. (c), Tumor volume was measured every seven days. (d), After 28 days of inoculation, the tumor weight of each group was examined. (e), Western blot was performed to detect the protein levels of PCNA in tumor tissues of xenograft BALB/c nude mice. (f), BALB/c nude mice were injected with NCI-H1299 cells with or without sh-FARSA using the tail vein containing 5 × 10⁵ cells in 0.1 mL PBS. Lung tissues were analyzed by H&E staining, Scale bar = 50 μm. **P < 0.01, ***P < 0.001, Compared with the sh-NC group.