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. 2022 Aug 3;21(24):2575–2589. doi: 10.1080/15384101.2022.2105087

Figure 4.

Figure 4.

CircFARSA acted as a sponge of miR-15a-5p. (a), Wild-type (WT) and mutated-type (MUT) sequences of the putative binding sites between circFARSA and miR-15a-5p. (b), qRT-PCR was applied to detect the expression of miR-15a-5p in 40 paired NSCLC tumor tissues and matched adjacent tissues; (c), The expression of miR-15a-5p in BEAS-2B and both NSCLC cell lines was examined using qRT-PCR. (d), The correlation between the expression of circFARSA and miR-15a-5p in tumor tissues of 40 NSCLC patients was analyzed by Spearman’s correlation coefficient. (e-g), The interaction between circFARSA and miR-15a-5p was verified using dual-luciferase reporter gene assay (e) and RNA immunoprecipitation (f and g). (h), qRT-PCR was employed to examine the expression of miR-15a-5p in both NSCLC cells after transfection with circFARSA shRNA, circFARSA overexpression plasmid, and corresponding negative control. (i), The FISH assay was used to detect the colocalization of circFARSA and miR-15a-5p in both NSCLC cells. **P < 0.01, ***P < 0.001.