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. 2022 Aug 3;21(24):2575–2589. doi: 10.1080/15384101.2022.2105087

Figure 5.

Figure 5.

B7H3 was verified as a target for miR‑15a‑5p. (a), WT and MUT sequences of the putative binding sites between B7H3 and miR-15a-5p. (b), The mRNA levels of B7H3 in NSCLC tissues (n = 486) and normal tissues (n = 338) were analyzed from the GEPIA database. (c), qRT-PCR was performed to examine the mRNA expression of B7H3 in NSCLC tumor tissues (n = 40) and matched adjacent normal tissues (n = 40). (d), Western blot was used to measure the protein levels of B7H3 in NSCLC cell lines and BEAS-2B cells. Spearman’s correlation coefficient was applied to analyze the correlation between B7H3 and miR-15a-5p (e) or circFARSA (f) in 40 NSCLC tumor tissues. (g), Association between B7H3 expression and overall survival rate of patients with NSCLC was analyzed using Kaplan-Meier curves analysis. (h), Dual-luciferase reporter gene assay was employed to verify the target association between B7H3 and miR-15a-5p in 293 T cells. (i and j), The protein level of B7H3 in transfected NSCLC cells was determined using western blot. *P < 0.05, ***P < 0.001.