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. 2022 Aug 16;21(24):2602–2614. doi: 10.1080/15384101.2022.2105885

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Figure 3. — METTL3 enhanced the stability of DLGAP1-AS2 in m⁶A-dependent manner. (a) GEPIA dataset revealed the positive correlation within METTL3 and DLGAP1-AS2 in LUSC (Lung squamous cell carcinoma, http://gepia.cancer-pku.cn/). (b) The METTL3 protein was detected in NSCLC cells upon METTL3 overexpression transfection. (c, d) The m⁶A modification levels were detected using m⁶A RNA Methylation Quantification Kit (Colorimetric). (e, f) RNA stability analysis revealed the RNA remaining of DLGAP1-AS2 in H1299 cells and A549 cells treated by Act D (actinomycin D, 1 μg/m). LncRNA DLGAP1-AS2 was determined by qRT-PCR assay. *p < 0.05.