Figure 4.

DLGAP1-AS2 promoted the aerobic glycolysis of NSCLC. (a) Detection of DLGAP1-AS2 levels by qRT-PCR in NSCLC cell lines (H1299, A549) respectively transfected with DLGAP1-AS2 overexpression plasmids or lentivirus shRNA. (b) Glucose uptake quantity was quantificationally detected using glucose uptake kit in H1299 and A549 cells. (c) Lactate production was quantificationally detected using lactate analysis kit. (d) ATP quantitative analysis was performed in H1299 or A549 cells. (e, f) The glycolysis levels of H1299 and A549 was detected using a seahorse analyzer. (g) In vivo animal experiments were performed to test the tumor neoplasm growth of NSCLC cells (A549) transfected with DLGAP1-AS2 silencing (sh-DLGAP1-AS2). The data are presented as the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.